Issue 14, 2022

Recent advances in microfluidic-based electroporation techniques for cell membranes

Abstract

Electroporation is a fundamental technique for applications in biotechnology. To date, the ongoing research on cell membrane electroporation has explored its mechanism, principles and potential applications. Therefore, in this review, we first discuss the primary electroporation mechanism to help establish a clear framework. Within the context of its principles, several critical terms are highlighted to present a better understanding of the theory of aqueous pores. Different degrees of electroporation can be used in different applications. Thus, we discuss the electric factors (shock strength, shock duration, and shock frequency) responsible for the degree of electroporation. In addition, finding an effective electroporation detection method is of great significance to optimize electroporation experiments. Accordingly, we summarize several primary electroporation detection methods in the following sections. Finally, given the development of micro- and nano-technology has greatly promoted the innovation of microfluidic-based electroporation devices, we also present the recent advances in microfluidic-based electroporation devices. Also, the challenges and outlook of the electroporation technique for cell membrane electroporation are presented.

Graphical abstract: Recent advances in microfluidic-based electroporation techniques for cell membranes

Associated articles

Article information

Article type
Critical Review
Submitted
05 Feb 2022
Accepted
15 Jun 2022
First published
01 Jul 2022

Lab Chip, 2022,22, 2624-2646

Recent advances in microfluidic-based electroporation techniques for cell membranes

F. Wang, S. Lin, Z. Yu, Y. Wang, D. Zhang, C. Cao, Z. Wang, D. Cui and D. Chen, Lab Chip, 2022, 22, 2624 DOI: 10.1039/D2LC00122E

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