Metal-dependent glycosylation in recombinant metallothioneins

Mario García-Risco; Ana González; Sara Calatayud; Francisco J. Lopez-Jaramillo; Veronika Pedrini-Martha; Ricard Albalat; Reinhard Dallinger; José M. Dominguez-Vera; Òscar Palacios; Mercè Capdevila

doi:10.1039/D2CC05589A

Metal-dependent glycosylation in recombinant metallothioneins

Mario García-Risco,

^a Ana González,

^b Sara Calatayud,^c Francisco J. Lopez-Jaramillo,

^d Veronika Pedrini-Martha,^e Ricard Albalat,

^cf Reinhard Dallinger,

^e José M. Dominguez-Vera,

*^b Òscar Palacios

^a and Mercè Capdevila

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, Universitat Autònoma de Barcelona, Cerdanyola del Vallès (E-08193), Spain
E-mail: merce.capdevila@uab.cat

^b Department of Inorganic Chemistry and Biotechnology Institute, University of Granada, Granada (E-18071), Spain
E-mail: josema@ugr.es

^c Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona (E-08028), Spain

^d Department of Organic Chemistry and Biotechnology Institute, University of Granada, Granada (E-18071), Spain

^e Institute of Zoology and Center of Molecular Biosciences, University of Innsbruck, Innsbruck (A-60210), Austria

^f Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona (E-08028), Spain

Abstract

We show for the first time glycosylation of recombinant metallothioneins (MTs) produced in E. coli. Interestingly, our results show that the glycosylation level of the recombinant MTs is inversely proportional to the degree of protein structuration, and reflects their different metal preferences.

Chemical Communications

Metal-dependent glycosylation in recombinant metallothioneins

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Metal-dependent glycosylation in recombinant metallothioneins

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