Semi-quantification of the binding constant based on bond breaking in a combined acoustic–gravitational field

Abstract

In this study, we propose a semi-quantification method based on breaking bonds between microparticles and glass plates in a combined acoustic–gravitational field. The semi-quantified binding constant values for BSA–ibuprofen, BSA–ciprofloxacin, ConA–glycogen, ConA–mannan, and BSA–naproxen calculated using this method were 7.5 × 10³, 1.6 × 10⁴, 2.3 × 10⁵, 2.4 × 10⁶ and 9.0 × 10⁷ M⁻¹, respectively, which were in concord with the reported values.