Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange

Abstract

Residues of flumetralin, a dinitroaniline herbicide, in foods pose a health risk to humans. In this study, we produced a sensitive monoclonal antibody (mAb) against flumetralin and developed a gold nanoparticle-based lateral flow immunoassay (LFIA) strip for screening flumetralin in orange. The subtype of mAb 3F5 was IgG1 and had a half-maximal inhibitory concentration of 9.43 ng mL⁻¹. The cross-reactivity assay indicated that mAb had good specificity. The LFIA strip had a visual limit of detection of 200 ng g⁻¹ in orange. We obtained results with the naked eye within 15 min. The calculated LOD of the strip in orange was 33.26 ng g⁻¹, with a linear detection of 80.72–1672.10 ng g⁻¹. The strip had recovery rates ranging from 98.4% to 110.1%. Therefore, our developed method is an effective and reliable tool for the rapid detection of flumetralin in orange.