We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.
T. Kondo, M. Eguchi, S. Kito, T. Fujino, G. Hayashi and H. Murakami, Chem. Commun., 2021, 57, 2416 DOI: 10.1039/D0CC07541H
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