The fabrication of a thiol-modified chitosan magnetic graphene oxide nanocomposite and its adsorption performance towards the illegal drug clenbuterol in pork samples

Abstract

A novel thiol (provided by (3-mercaptopropyl) trimethoxysilane, MPTS)-modified chitosan magnetic graphene oxide nanocomposite (Fe₃O₄@SiO₂/GO/CS/MPTS) was synthesized and characterized for the first time as an efficient magnetic sorbent for the enrichment and extraction of trace levels of clenbuterol in pork samples (muscle, fat, heart and liver). Various greatly influential parameters were optimized using a Box-Behnken design (BBD) through the response surface methodology (RSM) to obtain more satisfactory recovery. Under optimum conditions, the method detection limits (MDLs) were in the range of 0.054–0.136 ng g⁻¹. The recoveries of three spiked levels ranged from 84.7% to 101.1%, and the relative standard deviations (RSDs) were lower than 9.3%. The results of the adsorption experiments showed that the maximum adsorption capacity of Fe₃O₄@SiO₂/GO/CS/MPTS for clenbuterol was 214.13 mg g⁻¹. The adsorption process was most consistent with pseudo second-order kinetics and Langmuir adsorption isotherm, indicating a homogeneous process with a chemisorptive nature. Also, the nanocomposite exhibited high adsorption capability for clenbuterol compared with Fe₃O₄@SiO₂/GO and Fe₃O₄@SiO₂/GO/CS. In addition, regeneration of the nanocomposite was effectively achieved, and it retained about 82% of its initial capacity after four cycles. All these results indicate that the synthetic nanocomposite is a promising efficient adsorbent for the adsorption of clenbuterol with high adsorption capacity and low cost.