A systematic study on the discrepancy of fluorescence properties between in solutions and in cells: super-bright, environment-insensitive benzocoumarin dyes

Ye Jin Reo; Yong Woong Jun; Seo Won Cho; Jinseong Jeon; Hajung Roh; Subhankar Singha; Mingchong Dai; Sourav Sarkar; Hye Rim Kim; Sojeong Kim; Yusung Jin; Yun Lim Jung; Yun Jae Yang; Changil Ban; Jinmyoung Joo; Kyo Han Ahn

doi:10.1039/D0CC03586F

A systematic study on the discrepancy of fluorescence properties between in solutions and in cells: super-bright, environment-insensitive benzocoumarin dyes†

Ye Jin Reo,

^a Yong Woong Jun,

*^b Seo Won Cho,^a Jinseong Jeon,^a Hajung Roh,^c Subhankar Singha,^d Mingchong Dai,

^a Sourav Sarkar,^a Hye Rim Kim,^a Sojeong Kim,^ac Yusung Jin,^c Yun Lim Jung,^a Yun Jae Yang,^a Changil Ban,

^a Jinmyoung Joo

*^ce and Kyo Han Ahn

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 37673, Republic of Korea
E-mail: ahn@postech.ac.kr

^b Department of Chemistry, Stanford University, Stanford, CA 94305, USA

^c Department of Convergence Medicine, Biomedical Engineering Research Center, Asan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea

^d Institute of Advanced Studies and Research, JIS University, Kolkata 700091, India

^e Department of Biomedical Engineering, School of Life Science, UNIST, 50 UNIST-gil, Ulsan 44919, Republic of Korea

Abstract

The benzocoumarin dyes fluoresce negligibly in aqueous media but very strongly in cells, whereas representative conventional dyes display contrasting behaviour; the distinct emission behaviour of the fluorophores in organic solutions, in aqueous media, and in cell convinces the uniqueness of the cellular environment. The in cellulo superbright benzocoumarins also reveal an environment-insensitive emission behaviour, which is required for the reliable analysis via ratiometric imaging.

Chemical Communications

A systematic study on the discrepancy of fluorescence properties between in solutions and in cells: super-bright, environment-insensitive benzocoumarin dyes†

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A systematic study on the discrepancy of fluorescence properties between in solutions and in cells: super-bright, environment-insensitive benzocoumarin dyes

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