Stem–loop clutch probes for sequence-specific dsDNA analysis with improved single-mismatch selectivity

Abstract

A stem–loop clutch probe (SLCP)-based strategy has been explored to guide sequence-specific double stranded DNA (dsDNA) analysis with enhanced single-base mismatch selectivity. This assay method can also modulate the dynamic range by employing natural processes relying on distal-site mutation inhibition and allosteric activation.