Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site

Fu Chang; Yueying Sun; Dandan Yang; Wenjuan Yang; Yuanyuan Sun; Chenghui Liu; Zhengping Li

doi:10.1039/C9CC02700A

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Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site†

Fu Chang,‡^a Yueying Sun,‡^ab Dandan Yang,^a Wenjuan Yang,^c Yuanyuan Sun,^a Chenghui Liu

*^a and Zhengping Li

*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, Shaanxi Province, P. R. China
E-mail: liuch@snnu.edu.cn, lzpbd@snnu.edu.cn
Fax: +86 29 81530859
Tel: +86 29 81530859

^b Key Laboratory of Molecular Biology and Antibody Drug Engineering, School of Life Sciences, Liao Cheng University, P. R. China

^c Department of Ultrasound, Xijing Hospital Affiliated to The Fourth Military Medical University, P. R. China

Abstract

A chimeric DNA probe-aided RNase H-EXPAR approach is developed for the accurate and specific detection of RNA mutation at single-base resolution through a new site-specific RNase H cleavage mechanism.

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Article information

DOI: https://doi.org/10.1039/C9CC02700A
Article type: Communication
Submitted: 08 Apr 2019
Accepted: 21 May 2019
First published: 21 May 2019

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Chem. Commun., 2019,55, 6934-6937

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Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site

F. Chang, Y. Sun, D. Yang, W. Yang, Y. Sun, C. Liu and Z. Li, Chem. Commun., 2019, 55, 6934 DOI: 10.1039/C9CC02700A

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Chemical Communications

Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site†

Abstract

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Article information

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Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site

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