Electrochemical sensing of attomolar miRNA combining cascade strand displacement polymerization and reductant-mediated amplification

Abstract

Herein, we have developed a distinctive electrochemical sensing platform for miRNA assay. Target miRNA initiates two cycles of polymerization, nicking, and displacement reactions, which lead to the variation of the distance between redox molecules and the electrode surface. In addition, a reductant is employed for further electrochemical amplification.