Issue 35, 2017

Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

Abstract

Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42–208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide and peptidoglycan layers, direct membrane disruption of both Gram-negative and Gram-positive bacteria is suppressed. However, LDH nanoparticles cause size-dependent charge reversal and resulting flocculation of both liposomes and bacteria, which may provide a mechanism for bacterial confinement or clearance. Taken together, these findings demonstrate a set of previously unknown behaviors, including synergistic membrane destabilization and dual confinement/killing of bacteria through combined LDH/AMP exposure, of potential therapeutic interest.

Graphical abstract: Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
25 Apr 2017
Accepted
28 Jun 2017
First published
06 Jul 2017

Phys. Chem. Chem. Phys., 2017,19, 23832-23842

Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

S. Malekkhaiat Häffner, L. Nyström, R. Nordström, Z. P. Xu, M. Davoudi, A. Schmidtchen and M. Malmsten, Phys. Chem. Chem. Phys., 2017, 19, 23832 DOI: 10.1039/C7CP02701J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements