Issue 22, 2017

Real-time assay of inorganic pyrophosphatase activity in the red region based on Li+-doped NaYF4:Yb,Er upconversion luminescent nanoparticles

Abstract

Li+-Doped NaYF4:Yb,Er upconversion luminescent nanoparticles (UCLNPs) were synthesized and modified with poly(acrylic acid) (PAA). The luminescence of the carboxyl-functionalized UCLNPs at 664 nm was quenched in the presence of cupric ions (Cu2+) due to the coordination of Cu2+ with the carboxyl group of PAA. When pyrophosphate ions (PPi) were added, the luminescence of the UCLNPs was restored due to the competitive binding of PPi with Cu2+. However, pyrophosphatase (PPase) could promote the hydrolysis of PPi, thus releasing Cu2+, leading to requenching of the luminescence. Under optimal conditions, the luminescence response was virtually proportional to the activity of the PPase between 0.500 and 50.0 mU mL−1, and the limit of detection was 0.441 mU mL−1. Based on the competition assay approach, a new highly sensitive method in the red region for real-time detection of PPase activity was developed.

Graphical abstract: Real-time assay of inorganic pyrophosphatase activity in the red region based on Li+-doped NaYF4:Yb,Er upconversion luminescent nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
20 Feb 2017
Accepted
04 May 2017
First published
08 May 2017

Anal. Methods, 2017,9, 3296-3301

Real-time assay of inorganic pyrophosphatase activity in the red region based on Li+-doped NaYF4:Yb,Er upconversion luminescent nanoparticles

L. Wang, Y. Ji, Y. He, L. Wang and H. Chen, Anal. Methods, 2017, 9, 3296 DOI: 10.1039/C7AY00444C

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