Issue 27, 2017

Quantitative analysis of 3-hydroxybenzo[a]pyrene and (+)-anti-benzo[a]pyrene-diolepoxide-DNA adducts in benzo[a]pyrene induced mice by the protection of lemongrass essential oil

Abstract

Benzo[a]pyrene (B[a]P) is a well-known carcinogen present in the environment. In this paper, we developed a method for determining the B[a]P intermediate metabolites, 3-hydroxybenzo(a)pyrene(3-OHB[a]P) and (+)-anti-benzo(a)pyrene diolepoxide-DNA adducts (BPDE-DNA) in the plasma, blood, brain and lung tissues of B[a]P-induced mice using high performance liquid chromatography coupled with fluorescence detection (HPLC-FD) and ultraviolet spectrophotometry. Besides, the protective effects of lemongrass essential oil (LEO) against B[a]P-induced mice damage were investigated using the developed method. SPF mice were randomly divided into 8 groups, dosed 50 mg kg−1 b.w. B[a]P of body weight, and each was fed 0.2 mL every week. LEO was in three concentrations of 0.05%, 0.1%, 0.25% aqueous solutions, inhaled for 30 min every day. After B[a]P exposure for 3 months, specimens from the plasma, blood, lung tissues and brain tissues were collected, extracted, and 3-OHB[a]P and benzo[a]pyrene-tetrol I-1 (B[a]P-tetrol I-1) completely released after acid hydrolysis and the (+)-anti-BPDE-DNA in the samples was measured. The calibration curves were linear (R2 > 0.9958). Intra-and inter-day assay data suggested that the method was accurate and precise. The recoveries and stability were stable under all conditions investigated. After the protection of LEO, the level of 3-OHB[a]P also increased and the level of (+)-anti-BPDE-DNA decreased in the plasma, blood, brain and lung tissues of B[a]P-induced mice. Our paper provides a simple, sensitive, reliable, rapid and low-cost method for the determination of 3-OHB[a]P and (+)-anti-BPDE-DNA in the samples of B[a]P-induced mice by the protection of LEO. The LEO had the effects of protecting damage induced by B[a]P in mice.

Graphical abstract: Quantitative analysis of 3-hydroxybenzo[a]pyrene and (+)-anti-benzo[a]pyrene-diolepoxide-DNA adducts in benzo[a]pyrene induced mice by the protection of lemongrass essential oil

Article information

Article type
Paper
Submitted
27 Mar 2017
Accepted
17 Jun 2017
First published
21 Jun 2017

Anal. Methods, 2017,9, 4091-4100

Quantitative analysis of 3-hydroxybenzo[a]pyrene and (+)-anti-benzo[a]pyrene-diolepoxide-DNA adducts in benzo[a]pyrene induced mice by the protection of lemongrass essential oil

N. Chang, H. Xu, N. Ma, Y. Ma, M. Fang, J. Cao and F. Li, Anal. Methods, 2017, 9, 4091 DOI: 10.1039/C7AY00809K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements