The antioxidative effects of three lactobacilli on high-fat diet induced obese mice

Abstract

In this paper, three Lactobacillus strains (L. coryniformis subsp. torquens T3, L. paracasei subsp. paracasei M5 and L. paracasei subsp. paracasei X12) isolated in our laboratory were investigated for antioxidant activity in vitro and in vivo. Hydroxyl and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities and reducing power were examined in vitro. Three Lactobacillus strains were administered to high-fat diet induced obese mice. The results indicated that T3 and M5 showed the highest hydroxyl radical and DPPH scavenging activities at a dose of 10⁹ CFU mL⁻¹, with inhibition rates of 40.33, 41.11% and 40.76, 44.31%, respectively. The reducing activities of these two strains were also significantly higher than those of X12. Compared with the high-fat diet group, M5 and T3 have effectively controlled body weight and hepatomegaly and significantly decreased blood triglyceride and total cholesterol levels (p < 0.05). Besides, M5 and T3 obviously improved the antioxidative indexes including MDA levels, T-SOD, GSH-Px, CAT and T-AOC activities. Overall, M5 and T3 have a strong antioxidant activity in vitro and can improve oxidative damage and control body weight in mice, suggesting that they may be potential anti-obesity and antioxidant probiotics.