Issue 23, 2016
From the journal:

Chemical Communications

Hairpin ribozyme mediated RNA recombination†

Robert Hieronymus,a   Simon Peter Godehard,a   Darko Balkea  and  Sabine Müller*a  

* Corresponding authors

a Ernst-Moritz-Arndt-Universität Greifswald, Institut für Biochemie, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany
E-mail: smueller@uni-greifswald.de

Abstract

We have engineered a hairpin ribozyme that in a two-step reaction supports RNA recombination by catalysing the cleavage of two non-functional RNA precursors (first step) followed by the recombination of two of the cleavage fragments into a functional RNA (second step). The recombination product (yield: 76%) is a fully functional hammerhead ribozyme.

Graphical abstract: Hairpin ribozyme mediated RNA recombination

  • This article is part of the themed collection: Foldamers
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Article information

DOI
https://doi.org/10.1039/C6CC00383D
Article type
Communication
Submitted
14 Jan 2016
Accepted
16 Feb 2016
First published
16 Feb 2016

Chem. Commun., 2016,52, 4365-4368

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Hairpin ribozyme mediated RNA recombination

R. Hieronymus, S. P. Godehard, D. Balke and S. Müller, Chem. Commun., 2016, 52, 4365 DOI: 10.1039/C6CC00383D

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