Issue 56, 2016, Issue in Progress
From the journal:

RSC Advances

Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates†

R. S. Tan,*ab   H. Hinoucd  and  S.-I. Nishimuracd  

* Corresponding authors

a Department of Chemistry, College of Science, De La Salle University, 2401 Taft Ave., Manila 1004, Philippines
E-mail: roger.tan@dlsu.edu.ph

b Microalgae Systematics and Applied Phycology Research Unit, Center for Natural Science & Environmental Research (CENSER), De La Salle University, 2401 Taft Ave., Manila 1004, Philippines

c Graduate School of Life Science, Research Center for Post-Genome Science and Technology, Hokkaido University, N21, W10, Kita-ku, Sapporo 001-0021, Japan

d Medicinal Chemistry Pharmaceuticals Co. Ltd., N21, W12, Kita-ku, Sapporo 001-0021, Japan

Abstract

A novel β-galactosynthase–β-mannosynthase dual-activity of β-galactosidase from Aspergillus oryzae was uncovered, for the first time, using free monosaccharide substrates. The enzyme successfully converted galactose and mannose monomer sugars efficiently into Gal-β(1–6)-Gal and Man-β(1–6)-Man, respectively. The discovery could potentially revolutionize chemoenzymatic glycan and non-digestible oligosaccharide (NDO) syntheses.

Graphical abstract: Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates

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Article information

DOI
https://doi.org/10.1039/C6RA08060J
Article type
Communication
Submitted
29 Mar 2016
Accepted
17 May 2016
First published
18 May 2016

RSC Adv., 2016,6, 50833-50836

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Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates

R. S. Tan, H. Hinou and S.-I. Nishimura, RSC Adv., 2016, 6, 50833 DOI: 10.1039/C6RA08060J

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