Noninvasive target CT detection and anti-inflammation of MRSA pneumonia with theranostic silver loaded mesoporous silica

Abstract

In this study, the non-invasive diagnosis of methicillin resistant Staphylococcus aureus (MRSA) related pneumonia via X-ray CT and related anti-inflammatory profiles post theranostic silver loaded mesoporous silica nanomedicine is reported. Mesoporous silica NPs were firstly synthesized and chemically modified to covalently bond to silver clusters and anti-MRSA antibodies. The structure of the hybrid was investigated via transmission electron microscopy, dynamic light scattering analysis and X-ray photoelectron spectra. MTT and broth dilution assays confirmed the biocompatibility and antibacterial potency of the platform. In vivo CT study of rats bearing MRSA pneumonia and anti-inflammatory profiles as bronchoalveolar lavage and pathological assays give evidence to the theranostic potential of our platform. Biochemical and hematological assays confirmed the long term safety of the nanomedicine for in vivo application.