A new ratiometric fluorescent probe for rapid, sensitive and selective detection of endogenous hydrogen sulfide in mitochondria

Abstract

We have developed a new ratiometric fluorescent probe composed of a coumarin–merocyanine dyad based on the FRET mechanism. The probe showed clear dual-emission signal changes in blue and red spectral windows upon addition of H₂S in a dose dependent manner under a single wavelength excitation. The probe targeted mitochondria with high selectivity and sensitivity toward H₂S.