We developed a novel strategy by oxidation–derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.
H. Zhang, J. Xiong, B. Qi, Y. Feng and B. Yuan, Chem. Commun., 2016, 52, 737 DOI: 10.1039/C5CC07354E
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