Dye-conjugated upconversion nanoparticles for ratiometric imaging of intracellular pH values

Tingchun Ma; Yun Ma; Shujuan Liu; Lili Zhang; Tianshe Yang; Hui-Ran Yang; Wen Lv; Qi Yu; Wenjuan Xu; Qiang Zhao; Wei Huang

doi:10.1039/C5TC00849B

Dye-conjugated upconversion nanoparticles for ratiometric imaging of intracellular pH values†

Tingchun Ma,‡^a Yun Ma,‡^a Shujuan Liu,^a Lili Zhang,^a Tianshe Yang,^a Hui-Ran Yang,^a Wen Lv,^a Qi Yu,^a Wenjuan Xu,^a Qiang Zhao*^a and Wei Huang*^ab

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* Corresponding authors

^a Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials, National Jiangsu Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications (NUPT), Nanjing 210023, P. R. China
E-mail: iamqzhao@njupt.edu.cn

^b Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), Nanjing 211816, P. R. China
E-mail: wei-huang@njtech.edu.cn

Abstract

A ratiometric pH probe based on Tm³⁺ doped UCNPs functionalized with dye xylenol orange on the surface was developed, which can realize ratiometric pH sensing and imaging under continuous-wave excitation at 980 nm through the quenching and recovery of upconversion luminescence at 450 nm from UCNPs.

Journal of Materials Chemistry C

Dye-conjugated upconversion nanoparticles for ratiometric imaging of intracellular pH values†

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Dye-conjugated upconversion nanoparticles for ratiometric imaging of intracellular pH values

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