Issue 4, 2015

Time-resolved fluorescence anisotropy as a tool to study guest–cucurbit[n]uril–protein ternary supramolecular interactions

Abstract

Ternary supramolecular complexes involving cucurbit[n]urils and proteins are of potential interest for improving drug transport and delivery. We report here time-resolved fluorescence studies for acridine orange complexes with cucurbit[7]uril and cucurbit[8]uril in the presence of human serum albumin as a model system. A detailed characterization of the fluorescence lifetime and anisotropy properties of the different acridine orange complexes with cucurbit[n]urils and human serum albumin was performed. Of particular importance is the analysis of the stepwise binding for acridine orange–cucurbit[8]uril complexes and the assignment of the fluorescence and anisotropy properties to the 2 : 1 complex. Anisotropy decay measurements were essential to detect protein-bound species and to discriminate between different complexes. Based on the fluorescence evidence, ternary interactions with the protein are suggested for the acridine orange–cucurbit[7]uril complex but not for the cucurbit[8]uril complex. We highlight here the usability and sensitivity of the combined fluorescence analysis.

Graphical abstract: Time-resolved fluorescence anisotropy as a tool to study guest–cucurbit[n]uril–protein ternary supramolecular interactions

Supplementary files

Article information

Article type
Paper
Submitted
16 Dec 2014
Accepted
22 Jan 2015
First published
03 Feb 2015

Photochem. Photobiol. Sci., 2015,14, 842-852

Time-resolved fluorescence anisotropy as a tool to study guest–cucurbit[n]uril–protein ternary supramolecular interactions

K. Scholtbach, Í. Venegas, C. Bohne and D. Fuentealba, Photochem. Photobiol. Sci., 2015, 14, 842 DOI: 10.1039/C4PP00479E

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