A single nanoparticle-based sensor for hydrogen peroxide (H2O2) via cytochrome c-mediated plasmon resonance energy transfer

Yura Kim; Ji Youn Park; Hye Young Kim; Minzae Lee; Jongheop Yi; Inhee Choi

doi:10.1039/C5CC05327G

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A single nanoparticle-based sensor for hydrogen peroxide (H₂O₂) via cytochrome c-mediated plasmon resonance energy transfer†

Yura Kim,‡^a Ji Youn Park,‡^a Hye Young Kim,^a Minzae Lee,^b Jongheop Yi^b and Inhee Choi*^a

Author affiliations

* Corresponding authors

^a Nanobiointerface Laboratory, Department of Life science, University of Seoul, Dongdaemun-ku, Seoul 130-743, Republic of Korea
E-mail: inheechoi1@uos.ac.kr

^b Department of Chemical and Biological Engineering, Seoul National University, Gwanak-ku, Seoul 151-742, Republic of Korea

Abstract

Herein, we report a novel method for H₂O₂ detection based on a single plasmonic nanoprobe via cytochrome c (Cyt c)-mediated plasmon resonance energy transfer (PRET). Dynamic spectral changes were observed in the fingerprint quenching dip of a single plasmonic nanoprobe in response to changes in the redox state of Cyt c, induced by H₂O₂. Based on the changes in the spectral profile of the single plasmonic nanoprobe, H₂O₂ was successfully detected in a wide concentration range from 100 mM to 10 nM, including physiologically relevant micromolar and nanomolar concentrations.

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Article information

DOI: https://doi.org/10.1039/C5CC05327G
Article type: Communication
Submitted: 29 Jun 2015
Accepted: 21 Aug 2015
First published: 21 Aug 2015

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Chem. Commun., 2015,51, 15370-15373

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A single nanoparticle-based sensor for hydrogen peroxide (H₂O₂) via cytochrome c-mediated plasmon resonance energy transfer

Y. Kim, J. Y. Park, H. Y. Kim, M. Lee, J. Yi and I. Choi, Chem. Commun., 2015, 51, 15370 DOI: 10.1039/C5CC05327G

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