Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity

Abstract

A solid-phase strategy using lipase as a biomolecular scaffold to produce a large amount of Cu²⁺-metalloenzyme is proposed here. The application of this protocol on different 3D cavities of the enzyme allows creating a heterogeneous artificial metallolipase showing chimeric catalytic activity. The artificial catalyst was assessed in Diels–Alder cycloaddition reactions and cascade reactions showing excellent catalytic properties.