Issue 5, 2015
From the journal:

Analytical Methods

Detection of interaction between protein trytophan residues and small or macromolecular ligands by synchrotron radiation magnetic circular dichroism†

Ashley J. Hughes,‡ab   Timothy R. Rudd,ba   Gareth S. A. Wright,a   David G. Fernig,a   Giuliano Siligardi*ba  and  Edwin A. Yates*ac  

* Corresponding authors

a Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
E-mail: eayates@liv.ac.uk

b Diamond Light Source Ltd, Harwell Innovation Campus, Didcot, Oxfordshire OX11 8DE, UK
E-mail: giuliano.siligardi@diamond.ac.uk

c Departamento de Bioquímica, Universidade Federal de São Paulo (UNIFESP), Rua Três de Maio 100, Vila Clementino, São Paulo, Brazil

Abstract

Presented herein is a novel method for the detection of tryptophan–ligand interactions utilising magnetic circular dichroism. Two examples are presented, one a small molecule and another, a large polysaccharide, heparan sulfate. These data are also the first reported example of a Trp–glycosaminoglycan interaction shown by magnetic circular dichroism.

Graphical abstract: Detection of interaction between protein trytophan residues and small or macromolecular ligands by synchrotron radiation magnetic circular dichroism

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Article information

DOI
https://doi.org/10.1039/C4AY02618G
Article type
Communication
Submitted
03 Nov 2014
Accepted
09 Dec 2014
First published
11 Dec 2014

Anal. Methods, 2015,7, 1667-1671

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Detection of interaction between protein trytophan residues and small or macromolecular ligands by synchrotron radiation magnetic circular dichroism

A. J. Hughes, T. R. Rudd, G. S. A. Wright, D. G. Fernig, G. Siligardi and E. A. Yates, Anal. Methods, 2015, 7, 1667 DOI: 10.1039/C4AY02618G

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