Issue 67, 2014
From the journal:

Chemical Communications

Pyrazine-derived disulfide-reducing agent for chemical biology†

John C. Lukesh, III,a   Kelly K. Wallinb  and  Ronald T. Raines*ab  

* Corresponding authors

a Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA
E-mail: rtraines@wisc.edu

b Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1544, USA

Abstract

For fifty years, dithiothreitol (DTT) has been the preferred reagent for the reduction of disulfide bonds in proteins and other biomolecules. Herein we report on the synthesis and characterization of 2,3-bis(mercaptomethyl)pyrazine (BMMP), a readily accessible disulfide-reducing agent with reactivity under biological conditions that is markedly superior to DTT and other known reagents.

Graphical abstract: Pyrazine-derived disulfide-reducing agent for chemical biology

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Article information

DOI
https://doi.org/10.1039/C4CC04491F
Article type
Communication
Submitted
13 Jun 2014
Accepted
20 Jun 2014
First published
11 Jul 2014

Chem. Commun., 2014,50, 9591-9594

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Pyrazine-derived disulfide-reducing agent for chemical biology

J. C. Lukesh, III, K. K. Wallin and R. T. Raines, Chem. Commun., 2014, 50, 9591 DOI: 10.1039/C4CC04491F

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