Singlet oxygen and ROS in a new light: low-dose subcellular photodynamic treatment enhances proliferation at the single cell level

Abstract

Two-photon excitation of a sensitizer with a focused laser beam was used to create a spatially-localized subcellular population of reactive oxygen species, ROS, in single HeLa cells. The sensitizer used was protoporphyrin IX, PpIX, endogenously derived from 5-aminolevulinic acid delivered to the cells. Although we infer that singlet oxygen, O₂(a¹Δ_g), is one ROS produced upon irradiation of PpIX under these conditions, it is possible that the superoxide ion, O₂⁻˙, may also play a role in this system. With a “high” dose of PpIX-sensitized ROS, the expected death of the cell was observed. However, under “low dose” conditions, clear signs of cell proliferation were observed. The present results facilitate studies of ROS-mediated signalling in imaging-based single cell experiments.