A role for hydrogen bonding in DNA recognition by the non-classical CCHHC type zinc finger, NZF-1

Abstract

The non-classical zinc finger protein, Neural Zinc Finger Factor-1, contains six Cys₂His₂Cys domains. All three cysteines and the second histidine directly bind Zn(II). Using a combination of mutagenesis, metal coordination and DNA binding studies, we report that the first histidine is involved in a functionally important hydrogen bonding interaction.