Issue 98, 2014
From the journal:

Chemical Communications

A solid-phase affinity labeling method for target-selective isolation and modification of proteins†

Daichi Kuwahara,a   Takahiro Hasumi,a   Hajime Kaneko,a   Madoka Unno,a   Daisuke Takahashi*a  and  Kazunobu Toshima*a  

* Corresponding authors

a Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
E-mail: dtak@applc.keio.ac.jp, toshima@applc.keio.ac.jp
Fax: +81-45-566-1537

Abstract

Solid-phase affinity labeling of a target protein, peanut agglutinin (PNA), with the specifically designed chemical tool 1 selectively and effectively furnished the labeled PNA. Furthermore, this method was applicable to native human carbonic anhydrase II in red blood cell lysate using the chemical tool 2 without the need for tedious manipulations.

Graphical abstract: A solid-phase affinity labeling method for target-selective isolation and modification of proteins

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Article information

DOI
https://doi.org/10.1039/C4CC06783E
Article type
Communication
Submitted
28 Aug 2014
Accepted
23 Oct 2014
First published
23 Oct 2014

Chem. Commun., 2014,50, 15601-15604

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A solid-phase affinity labeling method for target-selective isolation and modification of proteins

D. Kuwahara, T. Hasumi, H. Kaneko, M. Unno, D. Takahashi and K. Toshima, Chem. Commun., 2014, 50, 15601 DOI: 10.1039/C4CC06783E

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