Issue 90, 2014
From the journal:

Chemical Communications

Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS†

Zhenzhen Deng,ab   Mingliang Ye,*a   Yangyang Bian,ab   Zheyi Liu,ab   Fangjie Liu,ab   Chunli Wang,ab   Hongqiang Qina  and  Hanfa Zou*a  

* Corresponding authors

a Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
E-mail: mingliang@dicp.ac.cn, hanfazou@dicp.ac.cn
Fax: +86-411-84379620
Tel: +86-411-84379620

b Graduate School of Chinese Academy of Sciences, Beijing 100049, China

Abstract

A simple, cost-effective and high throughput method was developed for multiplexed kinase activity assay based on the multiplex isotope labeling of designed substrate peptides. This strategy was successfully applied to monitor the time-dependent consumption of substrates and generation of products in the single and multiple substrate systems.

Graphical abstract: Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS

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Article information

DOI
https://doi.org/10.1039/C4CC04906C
Article type
Communication
Submitted
27 Jun 2014
Accepted
11 Sep 2014
First published
11 Sep 2014

Chem. Commun., 2014,50, 13960-13962

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Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS

Z. Deng, M. Ye, Y. Bian, Z. Liu, F. Liu, C. Wang, H. Qin and H. Zou, Chem. Commun., 2014, 50, 13960 DOI: 10.1039/C4CC04906C

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