Issue 59, 2014
From the journal:

Chemical Communications

A “turn on/off” scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy†

Chung-An Chen,a   Chun-Chi Wang,a   Yen-Ling Chen,b   Yuh-Jyh Jongcde  and  Shou-Mei Wu*af  

* Corresponding authors

a School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
E-mail: shmewu@kmu.edu.tw
Fax: +886-7-3159597
Tel: +886-7-3121101 ext. 2164

b Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

c Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

d Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

e College of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan

f Department of Chemistry, College of Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

Abstract

A “turn on/off” biosensor for diagnosis of exon 7 of the SMN1 gene was developed by employing a “scorpion primer”. This scorpion primer was based on the principle of fluorescence resonance energy transfer using a fluorophore, a blocker and a quencher. It was successfully applied to detect 10 volunteer samples, and not only to in vitro testing.

Graphical abstract: A “turn on/off” scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy

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Article information

DOI
https://doi.org/10.1039/C4CC03472D
Article type
Communication
Submitted
08 May 2014
Accepted
04 Jun 2014
First published
13 Jun 2014

Chem. Commun., 2014,50, 8069-8072

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A “turn on/off” scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy

C. Chen, C. Wang, Y. Chen, Y. Jong and S. Wu, Chem. Commun., 2014, 50, 8069 DOI: 10.1039/C4CC03472D

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