Toll-like receptor 3 modified Au electrodes: an investigation into the interaction of TLR3 immobilized on Au surfaces with poly(I:C)

Abstract

The rapid detection of viruses is crucial for medical diagnosis, environmental monitoring, public health, and homeland security. In this report, the development of a novel electrochemical impedance spectroscopy-based method utilizing Toll-Like Receptor 3 (TLR3)-modified Au electrodes for studying the interaction of TLR3 on a surface with polyinosinic–polycytidylic acid (poly(I:C)) as a dsRNA mimic (a molecular signature of viruses) has been described. The modified Au electrodes were prepared by covalent immobilization of TLR3 protein using lipoic acid N-hydroxysuccinimide ester (Lip-NHS) linkers. The electrochemical behavior of the modified electrodes and their applicability for sensing poly(I:C) was examined.