Microtubule nucleation from a functionalised SiO2 EM grid

Abhijit Saha; Indrani Chakraborty; Christian Kraft; Shashi Bhushan; Surajit Ghosh

doi:10.1039/C3RA40273H

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Microtubule nucleation from a functionalised SiO₂ EM grid†

Abhijit Saha,‡^a Indrani Chakraborty,‡^a Christian Kraft,^b Shashi Bhushan*^b and Surajit Ghosh*^a

Author affiliations

* Corresponding authors

^a Chemistry Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata-700032, West Bengal, India
E-mail: sghosh@iicb.res.in
Fax: +91-33-2473-5197/0284
Tel: +91-33-2499-5872

^b Rudolf-Virchow-Zentrum/DFG-Forschungszentrum für Experimentelle Biomedizin der Universität Würzburg, Josef-Schneider-Str. 2, Haus D15, D - 97080 Würzburg, Germany
E-mail: shashi.bhushan@virchow.uni-wuerzburg.de

Abstract

A novel Tris-NTA functionalised SiO₂ EM grid is generated through covalent chemical functionalisation. Here, we show immobilisation of functional oligohistidine-tagged proteins and nucleation of microtubules from microtubule polymerase immobilised on the SiO₂ EM grid surface.

Download options Please wait...

Supplementary files

Article information

DOI: https://doi.org/10.1039/C3RA40273H
Article type: Communication
Submitted: 18 Jan 2013
Accepted: 19 Mar 2013
First published: 20 Mar 2013

Download Citation

RSC Adv., 2013,3, 7688-7691

Permissions

Request permissions

Microtubule nucleation from a functionalised SiO₂ EM grid

A. Saha, I. Chakraborty, C. Kraft, S. Bhushan and S. Ghosh, RSC Adv., 2013, 3, 7688 DOI: 10.1039/C3RA40273H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

RSC Advances