Issue 9, 2013

Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

Abstract

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38–40%) rate was 50 μl min−1 giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7–100 ng ml−1 within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

Graphical abstract: Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

Article information

Article type
Paper
Submitted
17 Nov 2012
Accepted
27 Feb 2013
First published
27 Feb 2013

Lab Chip, 2013,13, 1790-1796

Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

A. A. Tajudin, K. Petersson, A. Lenshof, A.-M. Swärd-Nilsson, L. Åberg, G. Marko-Varga, J. Malm, H. Lilja and T. Laurell, Lab Chip, 2013, 13, 1790 DOI: 10.1039/C3LC41269E

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