Designer cells for a synthetic cascade reaction harnessing selective redox reactions were devised, featuring two successive regioselective P450-catalyzed CH-activating oxidations of 1-cyclohexene carboxylic acid methyl ester followed by stereoselective olefin-reduction catalysed by (R)- or (S)-selective mutants of an enoate reductase.
R. Agudo and M. T. Reetz, Chem. Commun., 2013, 49, 10914 DOI: 10.1039/C3CC46229C
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