Activity enhancement of Microperoxide-11 immobilized on nanospheres with a nanosize Co3O4 core and a periodic mesoporous organosilica shell

Abstract

Core-shell nanospheres with a nanosize Co₃O₄ core and a periodic mesoporous organosilica (PMO) shell were fabricated by the packing and self-assembly of nanosize Co₃O₄ particles–surfactant–organosilica complexes based on the S⁺I⁻ pathway under basic conditions. The amount of Co₃O₄ nanoparticles is crucial to the formation of core–shell nanospheres. The obtained nanospheres possess a uniform particle size of 130 nm, a high surface area of 474.1 m²g⁻¹ and a large pore volume of 0.68 cm³g⁻¹, which are highly advantageous to the immobilization of Microperoxidase-11 (MP-11). The presence of Co₃O₄ nanoparticles could enhance the activity of immobilized MP-11, probably due to their peroxidase activity.