Issue 21, 2012
From the journal:

Chemical Communications

Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)†

Musleh M. Muthana,‡a   Jingyao Qu,‡a   Yanhong Li,a   Lei Zhang,a   Hai Yu,a   Li Ding,ab   Hamed Malekana  and  Xi Chen*a  

* Corresponding authors

a Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA
E-mail: chen@chem.ucdavis.edu
Fax: +1 530-752-8995
Tel: +1 530-754-6037

b College of Science, Northwest A&F University, Yangling, Shaanxi, China

Abstract

A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further chemical diversification of a UDP-mannose derivative resulted in the formation of UDP-N-acetylmannosamine.

Graphical abstract: Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

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Article information

DOI
https://doi.org/10.1039/C2CC17577K
Article type
Communication
Submitted
05 Dec 2011
Accepted
09 Jan 2012
First published
10 Jan 2012

Chem. Commun., 2012,48, 2728-2730

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Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

M. M. Muthana, J. Qu, Y. Li, L. Zhang, H. Yu, L. Ding, H. Malekan and X. Chen, Chem. Commun., 2012, 48, 2728 DOI: 10.1039/C2CC17577K

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