Issue 22, 2012
From the journal:

Chemical Communications

Light-activatable molecular beacons with a caged loop sequence†

Khashti Ballabh Joshi,‡a   Andreas Vlachos,‡b   Vera Mikat,‡a   Thomas Dellerb  and  Alexander Heckel*a  

* Corresponding authors

a Goethe University Frankfurt, Frankfurt Institute for Molecular Life Sciences (FMLS), Max-von-Laue-Str. 15, 60438 Frankfurt (M), Germany
E-mail: heckel@uni-frankfurt.de
Fax: +49 (69) 798-763 42505
Tel: +49 (69) 798-42505

b Goethe University Frankfurt, Institute of Clinical Neuroanatomy, Neuroscience Center, 60590 Frankfurt (M), Germany
E-mail: t.deller@em.uni-frankfurt.de
Fax: +49 (69) 6301-6425
Tel: +49 (69) 6301-6900

Abstract

We have synthesized molecular beacons with caged nucleobases in the loop region. These constructs remain non-fluorescent in the presence of their target RNA but can be fully activated by light (366 or 405 nm) in vitro and in HEK293 cells. We suggest that this technology will be useful for single molecule RNA tracking in living cells.

Graphical abstract: Light-activatable molecular beacons with a caged loop sequence

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Article information

DOI
https://doi.org/10.1039/C2CC16654B
Article type
Communication
Submitted
26 Oct 2011
Accepted
21 Nov 2011
First published
24 Nov 2011

Chem. Commun., 2012,48, 2746-2748

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Light-activatable molecular beacons with a caged loop sequence

K. B. Joshi, A. Vlachos, V. Mikat, T. Deller and A. Heckel, Chem. Commun., 2012, 48, 2746 DOI: 10.1039/C2CC16654B

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