Issue 8, 2012

Quantitative spot test analysis of soluble tannin in green tea using a portable diffuse reflectometer

Abstract

The study proposes a low cost and very simple quantitative spot test technique for determination of soluble tannin in green tea samples using a portable diffuse reflectometer. The lab-made instrument was made of polytetrafluoroethylene (PTFE), using a light emitting diode (LED) for its light source, and a phototransistor as the detector. The proposed method measured diffuse reflectance from a complex formed by reaction between soluble tannin and ferrous tartrate in a buffered medium at pH 6.8. The spot test is a reliable and advantageous alternative technique because it uses fewer chemicals than the reference method. The study obtained good limits of detection (0.11 mg L−1), and precision (RSD = 0.9%), and at a 95% confidence level (paired t-test) no statistically significant difference between the results of either method was found.

Graphical abstract: Quantitative spot test analysis of soluble tannin in green tea using a portable diffuse reflectometer

Article information

Article type
Paper
Submitted
10 Apr 2012
Accepted
05 May 2012
First published
09 May 2012

Anal. Methods, 2012,4, 2329-2333

Quantitative spot test analysis of soluble tannin in green tea using a portable diffuse reflectometer

M. B. Lima, S. I. E. Andrade, I. S. Barreto, L. F. Almeida, E. C. Silva and M. C. U. Araújo, Anal. Methods, 2012, 4, 2329 DOI: 10.1039/C2AY25361E

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