Issue 9, 2012
From the journal:

Analytical Methods

Study of inhibitory effect of mercury(ii) ion on exonuclease iiiviagel electrophoresis and microfluidic electrophoresis†

Min Yuan,ab   Yonggang Zhu,*b   Xinhui Lou,*c   Chen Chen,bde   Gang Wei,e   Minbo Land  and  Jianlong Zhao*a  

* Corresponding authors

a State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Rd, Shanghai 200050, China
E-mail: jlzhao@mail.sim.ac.cn
Tel: +86 21 62511070-8703

b CSIRO Materials Science and Engineering, PO Box 56, Highett, VIC 3190, Australia
E-mail: yonggang.zhu@csiro.au
Tel: +61 3 95452791

c Department of Chemistry, Capital Normal University, 105 Xisanhuan North Rd, Beijing 100084, China
E-mail: xlou9999@yahoo.com

d Shanghai Key Laboratory for Functional Material Chemistry, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China

e CSIRO Materials Science and Engineering, PO Box 218, Lindfield, NSW 2070, Australia

Abstract

The effect of mercury(II) ion on the exonucleolytic activity of Exo III was investigated by using microchip-based microfluidic electrophoresis coupled with polyacrylamide gel electrophoresis. This is the first report of this inhibitory effect with qualitative and quantitative analysis. With a series concentration of Exo III, the inhibition was quantitatively examined at various concentrations of mercuric ion ranging from 0 to 1 μM. For a given concentration of Exo III, the inhibition increased with increasing concentration of mercuric ions, whereas increasing concentration of Exo III hindered the inhibition activity. The inhibiting effect of 1 μM Hg2+ dropped significantly from (84.7 ± 1.95)% to (3.9 ± 1.25)% when the concentration of Exo III was increased from 0.02 to 0.5 units per μL. This study expands the application of microfluidic analytical methods to biochemistry and lays the foundation for the development of environmental research and genetic studies.

Graphical abstract: Study of inhibitory effect of mercury(ii) ion on exonuclease iiivia gel electrophoresis and microfluidic electrophoresis

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Article information

DOI
https://doi.org/10.1039/C2AY25214G
Article type
Paper
Submitted
29 Feb 2012
Accepted
08 Jun 2012
First published
13 Jun 2012

Anal. Methods, 2012,4, 2846-2851

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Study of inhibitory effect of mercury(II) ion on exonuclease IIIvia gel electrophoresis and microfluidic electrophoresis

M. Yuan, Y. Zhu, X. Lou, C. Chen, G. Wei, M. Lan and J. Zhao, Anal. Methods, 2012, 4, 2846 DOI: 10.1039/C2AY25214G

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