Issue 21, 2011
From the journal:

Soft Matter

Dual Click reactions to micropattern proteins

Rebecca M. Broyer,ab   Eric Schopf,bc   Christopher M. Kolodziej,ab   Yong Chenbc  and  Heather D. Maynard*ab  

* Corresponding authors

a Department of Chemistry and Biochemistry, University of California, Los Angeles, California, USA
E-mail: maynard@chem.ucla.edu

b California Nanosystems Institute, University of California, Los Angeles, California, USA

c Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, California, USA

Abstract

In the study described in this report orthogonal Click reactions were utilized to immobilize two different proteins on surfaces side-by-side and in multilayer constructs. Alkyne- and azide-functionalized poly(ethylene glycol) hydrogel features were fabricated. Copper-catalyzed Huisgen 1,3 dipolar cycloaddition and oxime chemistry were employed to conjugate an azide-functionalized ubiquitin and oxoamide-modified myoglobin, respectively. Multicomponent patterning was verified by fluorescence imaging.

Graphical abstract: Dual Click reactions to micropattern proteins

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C1SM05991B
Article type
Paper
Submitted
27 May 2011
Accepted
09 Aug 2011
First published
30 Aug 2011

Soft Matter, 2011,7, 9972-9977

Permissions

Request permissions

Dual Click reactions to micropattern proteins

R. M. Broyer, E. Schopf, C. M. Kolodziej, Y. Chen and H. D. Maynard, Soft Matter, 2011, 7, 9972 DOI: 10.1039/C1SM05991B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements