Detection of        DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe

Jean-Louis H. A. Duprey; Zheng-yun Zhao; Dario M. Bassani; Jack Manchester; Joseph S. Vyle; James H. R. Tucker

doi:10.1039/C1CC11205H

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Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe†‡

Jean-Louis H. A. Duprey,^a Zheng-yun Zhao,^a Dario M. Bassani,^b Jack Manchester,^a Joseph S. Vyle^c and James H. R. Tucker*^a

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* Corresponding authors

^a School of Chemistry, University of Birmingham, Edgbaston, Birmingham, UK
E-mail: j.tucker@bham.ac.uk
Fax: +44 (0)121 414 4403
Tel: +44 (0)121 414 4422

^b Institut des Sciences Moléculaires, CNRS UMR 5255, Université Bordeaux 1, 351 Cours de la Libération, F-33405 Talence, France
E-mail: d.bassani@ism.u-bordeaux1.fr
Fax: +33 (0)5 4000 6158
Tel: +33 (0)5 4000 2827

^c School of Chemistry and Chemical Engineering, David Keir Building, Queen's University Belfast (QUB), Stranmillis Rd, Belfast BT9 5AG, UK
E-mail: j.vyle@qub.ac.uk
Fax: +44 (0)28 9097 6524
Tel: +44 (0)28 9097 548

Abstract

A fluorescent DNA probe containing an anthracene group attached via an anucleosidic linker can identify all four DNA bases at a single site as well as the epigenetic modification C/5-MeCvia a hybridisation sensing assay.

This article is part of the themed collection: Supramolecular Chemistry

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Article information

DOI: https://doi.org/10.1039/C1CC11205H
Article type: Communication
Submitted: 28 Feb 2011
Accepted: 19 Apr 2011
First published: 12 May 2011

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Chem. Commun., 2011,47, 6629-6631

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Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe

J. H. A. Duprey, Z. Zhao, D. M. Bassani, J. Manchester, J. S. Vyle and J. H. R. Tucker, Chem. Commun., 2011, 47, 6629 DOI: 10.1039/C1CC11205H

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