Issue 6, 2011
From the journal:

Molecular BioSystems

Characterization of TioQ, a type II thioesterase from the thiocoraline biosynthetic cluster†

Ahmed S. A. Mady,‡ab   Olga E. Zolova,‡a   María Álvarez San Millán,c   Germán Villamizar,c   Fernando de la Calle,d   Felipe Lombóc  and  Sylvie Garneau-Tsodikova*aef  

* Corresponding authors

a University of Michigan, Life Sciences Institute, 210 Washtenaw Ave, Ann Arbor, USA

b Utrecht University, Department of Pharmaceutical Sciences, Netherlands

c Universidad de Oviedo, Departamento de Biología Funcional e Instituto, Universitario de Oncología del Principado de Asturias, 33006 Oviedo, Spain

d PharmaMar, S.A., 28770 Colmenar Viejo, Spain

e University of Michigan, Department of Medicinal Chemistry in the College of Pharmacy, 210 Washtenaw Ave, Ann Arbor, USA
E-mail: sylviegt@umich.edu
Fax: +1 734-515-5521
Tel: +1 734-615-2736

f University of Michigan, Chemical Biology Doctoral Program, Ann Arbor, USA

Abstract

An antitumor agent thiocoraline is a thiodepsipeptide marine product derived from two Micromonospora sp. strains that inhibits protein synthesis by binding of its key 3-hydroxyquinaldic acid (3HQA) chromophores to duplex DNA. There are at least two potential pathways via which the 3HQA moiety could be biosynthesized from L-Trp. By biochemical characterization and by preparation of knockouts of an adenylation–thiolation enzyme, TioK, and of two type II thioesterases, TioP and TioQ, found in the thiocoraline biosynthetic gene cluster, we gained valuable insight into the pathway followed for the production of 3HQA.

Graphical abstract: Characterization of TioQ, a type II thioesterase from the thiocoraline biosynthetic cluster

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Article information

DOI
https://doi.org/10.1039/C1MB05044C
Article type
Paper
Submitted
03 Feb 2011
Accepted
15 Mar 2011
First published
11 Apr 2011

Mol. BioSyst., 2011,7, 1999-2011

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Characterization of TioQ, a type II thioesterase from the thiocoraline biosynthetic cluster

A. S. A. Mady, O. E. Zolova, M. Á. S. Millán, G. Villamizar, F. D. L. Calle, F. Lombó and S. Garneau-Tsodikova, Mol. BioSyst., 2011, 7, 1999 DOI: 10.1039/C1MB05044C

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