Issue 20, 2011

Structuring of supported hybrid phospholipid bilayers on electrodes with phospholipase A2

Abstract

The effect of a lipolytic enzyme, pork pancreatic phospholipase A2, on hybrid bilayer membranes was monitored using voltammetry, impedance spectroscopy and surface plasmon resonance. The hybrid bilayers were prepared by Langmuir–Schaefer transfer of lipid monolayers onto gold electrodes modified with self-assembled alkanethiol monolayers, or by liposome spreading. The electrodes were immersed in the phospholipase aqueous solution to allow adsorption of the enzyme and cleavage of the ester bond in the sn-2 position of phospholipids in the outer leaflet of the hybrid layers. The action of phospholipase A2 led to perforation of the lipid films. Impedance spectroscopy and surface plasmon resonance were used for monitoring enzyme adsorption, phospholipid hydrolysis and product desorption. The results obtained show that transport efficiency of an electroactive probe, ferrocyanate, and of an electroactive drug, doxorubicin, through the bilayer depends on the action of the enzyme; the state of the lipid layer covering the electrode surface depends on the latter as well. Cyclic voltammetry and electrochemical impedance spectroscopy were used to study this effect. The doxorubicin reduction/oxidation signals appearing at potentials close to those observed using a bare gold electrode indicated facilitated penetration of the drug into the layer. The results obtained were interpreted in terms of pore formation in the lipid matrix; phospholipase A2 can be considered as a nano-device for high precision perforation of the lipid layer.

Graphical abstract: Structuring of supported hybrid phospholipid bilayers on electrodes with phospholipase A2

Article information

Article type
Paper
Submitted
21 Oct 2010
Accepted
17 Mar 2011
First published
18 Apr 2011

Phys. Chem. Chem. Phys., 2011,13, 9716-9724

Structuring of supported hybrid phospholipid bilayers on electrodes with phospholipase A2

A. Więckowska, E. Jabłonowska, E. Rogalska and R. Bilewicz, Phys. Chem. Chem. Phys., 2011, 13, 9716 DOI: 10.1039/C0CP02229B

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