Issue 11, 2010
From the journal:

Soft Matter

Interaction of reducible polypeptidegene delivery vectors with supported lipid bilayers: pore formation and structure–function relationships†

Mahmoud Soliman,ad   Rujikan Nasanit,b   Stephanie Allen,a   Martyn C. Davies,a   Simon S. Briggs,c   Leonard W. Seymour,c   Jon A. Preeceb  and  Cameron Alexander*a  

* Corresponding authors

a School of Pharmacy, University of Nottingham, Nottingham, UK
E-mail: cameron.alexander@nottingham.ac.uk
Fax: +44 (0)115 951 5102
Tel: +44 (0)115 846 7678

b School of Chemistry, University of Birmingham, Birmingham, UK

c Department of Clinical Pharmacology, University of Oxford, Oxford, UK

d Department of Pharmaceutics, Faculty of Pharmacy, Ain Shams University, Monazamet El Wehda El Afrikia St, El Abbassia, Cairo, Egypt

Abstract

Real-time atomic force microscopy (AFM) in aqueous buffer has been used to probe interactions of synthetic disulfide-linked polypeptide gene delivery vectors with supported phospholipid bilayers. Disruption of the membranes was apparent in AFM, and the extent of surface heterogeneity and hole (pore) formation was evaluated by depth and area-profiling image analysis. The overall extent of membrane disruption varied with the reducible polycation peptide sequence and block structure and was found to be correlated with the overall degree of transgene expression in two representative cell lines.

Graphical abstract: Interaction of reducible polypeptide gene delivery vectors with supported lipid bilayers: pore formation and structure–function relationships

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Article information

DOI
https://doi.org/10.1039/B927204F
Article type
Paper
Submitted
23 Dec 2009
Accepted
01 Apr 2010
First published
27 Apr 2010

Soft Matter, 2010,6, 2517-2524

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Interaction of reducible polypeptide gene delivery vectors with supported lipid bilayers: pore formation and structure–function relationships

M. Soliman, R. Nasanit, S. Allen, M. C. Davies, S. S. Briggs, L. W. Seymour, J. A. Preece and C. Alexander, Soft Matter, 2010, 6, 2517 DOI: 10.1039/B927204F

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