Issue 6, 2010

Conjugation of RAFT-generated polymers to proteins by two consecutive thiol–ene reactions

Abstract

Well-defined temperature-responsive polymers were covalently conjugated to model proteins by two consecutive Michael addition thiolene reactions. Poly(N-isopropylacrylamide) (PNIPAM) prepared by reversible addition–fragmentation chain transfer (RAFT) polymerization was aminolyzed to yield thiol-terminated chains that were subsequently reacted with excess 1,8-bis-maleimidodiethyleneglycol. The resulting maleimide-terminated polymer was reacted with bovine serum albumin and ovalbumin to yield polymerprotein conjugates by a “grafting-to” approach. The thermoresponsive nature of PNIPAM was conferred to the conjugate, as demonstrated by dynamic light scattering analysis that indicated the formation of intermolecular aggregates at elevated temperatures.

Graphical abstract: Conjugation of RAFT-generated polymers to proteins by two consecutive thiol–ene reactions

Article information

Article type
Paper
Submitted
22 Jan 2010
Accepted
15 Feb 2010
First published
29 Mar 2010

Polym. Chem., 2010,1, 854-859

Conjugation of RAFT-generated polymers to proteins by two consecutive thiolene reactions

M. Li, P. De, H. Li and B. S. Sumerlin, Polym. Chem., 2010, 1, 854 DOI: 10.1039/C0PY00025F

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