Issue 12, 2009

Microfabricated curtains for controlled cell seeding in high throughput microfluidic systems

Abstract

A microfabricated cell curtain is presented that facilitates cellular assays. The cell curtain is defined as a poly(dimethylsiloxane) (PDMS) wall that extends from the ceiling of a cell culture microchamber to within microns of the chamber floor. Curtain use is demonstrated by observing monolayer human epidermal keratinocyte (HEK) colonies for 48 h longer than possible with non-curtained microfluidic chambers. The curtains were further characterized by integrating them into a 96 chamber high throughput microfluidic cell culture device. As proof of concept, this device was used to assay a range of ethanol dilutions spanning 0–22% in cell culture medium. Cells exposed to 12% ethanol or less for 30 min would recover to 85% viability at 24 h, while cells exposed to higher concentrations had viabilities below 10%. The data also showed that cells exposed to 6% ethanol or less grew in population size, 8% ethanol exposure stunted growth, and higher concentrations led to population loss. Curtain use permitted high initial cell seeding densities and increased the amount of time cells can be cultured compared to multi-well plates.

Graphical abstract: Microfabricated curtains for controlled cell seeding in high throughput microfluidic systems

Article information

Article type
Paper
Submitted
04 Nov 2008
Accepted
25 Feb 2009
First published
20 Mar 2009

Lab Chip, 2009,9, 1756-1762

Microfabricated curtains for controlled cell seeding in high throughput microfluidic systems

A. T. O'Neill, N. A. Monteiro-Riviere and G. M. Walker, Lab Chip, 2009, 9, 1756 DOI: 10.1039/B819622B

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