An in situ amperometric biosensor for the detection of vapours from explosive compounds

Abstract

An amperometric biosensor based on genetically-modified enzymes was used for the in situdetection of trace vapours from a number of explosive compounds. The vapour samples that were generated from a purpose-built vapour generator were collected and pre-concentrated using a trap able to concentrate samples at a rate of 60-fold per minute of sampling. The amperometric biosensor achieved a remarkably low vapour detection limit of 6 parts per trillion from a room temperature sample. The specific activity of the reported enzyme toward a number of explosive compounds was also confirmed using absorbance measurements.