Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

David Williamson; Martin J. Cann; David R. W. Hodgson

doi:10.1039/B712066D

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Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

David Williamson,^a Martin J. Cann^b and David R. W. Hodgson*^a

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^a Department of Chemistry, University Science Laboratories, South Road, Durham, UK
E-mail: d.r.w.hodgson@durham.ac.uk

^b School of Biological and Biomedical Sciences, University Science Laboratories, South Road, Durham, UK

Abstract

5′-Amino-5′-deoxyguanosine-5′-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5′-H₂N-RNA that can be conjugated to activated esters.

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Article information

DOI: https://doi.org/10.1039/B712066D
Article type: Communication
Submitted: 06 Aug 2007
Accepted: 19 Sep 2007
First published: 11 Oct 2007

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Chem. Commun., 2007, 5096-5098

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Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

D. Williamson, M. J. Cann and D. R. W. Hodgson, Chem. Commun., 2007, 5096 DOI: 10.1039/B712066D

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Chemical Communications

Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

Abstract

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Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

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