Selective assembly of specifically charged proteins on an electrochemically switched surface

Abstract

A low-density self-assembled monolayer (LD-SAM) surface was generated via covalent bonding between Au and the inclusion complex, cyclodextrin (CD) encapsulated 16-mercaptohexadecanoic acid, followed by the dissociation of CD. The resulted LD-SAM was characterized by cyclic voltammetry, QCM and MALDI-TOF-MS measurements. Electric potential triggered reversible switching property of the LD-SAM was observed by contact angle measurements. The selective assembly of charged proteins, avidin and streptavidin, on the LD-SAM surface, which was carried out under different applied potentials, was investigated by QCM measurements and fluorescence spectrometry.