Issue 11, 2002
From the journal:

Journal of the Chemical Society, Perkin Transactions 2

Direct detection of superoxide anion generated in C60-photosensitized oxidation of NADH and an analogue by molecular oxygen

Ikuo Nakanishi,*a   Kei Ohkubo,b   Shunsuke Fujita,b   Shunichi Fukuzumi,*b   Toshifumi Konishi,c   Mamoru Fujitsuka,c   Osamu Ito*c  and  Naoki Miyata*d  

* Corresponding authors

a Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiological Sciences, Inage, Chiba 263-8555, Japan
E-mail: nakanis@nirs.go.jp
Fax: +81-43-255-6819
Tel: +81-43-206-3131

b Department of Material and Life Science, Graduate School of Engineering, Osaka University, CREST, Japan Science and Technology Corporation, Suita, Osaka 565-0871, Japan
E-mail: fukuzumi@chem.eng.osaka-u.ac.jp
Fax: +81-6-6879-7370
Tel: +81-6879-7368

c Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, CREST, Japan Science and Technology Corporation, Sendai, Miyagi 980-8577, Japan
E-mail: ito@tagen.tohoku.ac.jp
Fax: +81-22-217-5608
Tel: +81-22-217-5608

d Department of Organic and Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467-8603, Japan
E-mail: miyata-n@phar.nagoya-cu.ac.jp
Fax: +81-52-836-3407
Tel: +81-52-836-3407

Abstract

Visible-light irradiation of poly(vinylpyrrolidone) (PVP)-solubilized C60 in water in the presence of NADH (dihydronicotinamide adenine dinucleotide) and molecular oxygen (O2) results in formation of superoxide anion (O2˙−). Formation of O2˙− having a characteristic g‖ value of 2.18 was evidenced by the direct observation with use of a low-temperature EPR technique at 77 K. Photoinduced O2˙− formation was also observed for an N-methyl-2-pyrrolidone (NMP) solution of C60 and 1-benzyl-1,4-dihydronicotinamide (BNAH) in the presence of O2, whereas C60 radical anion (C60˙−) was formed in the absence of O2 under otherwise the same experimental conditions. These results suggest that C602− formed in the photoinduced electron-transfer reduction of C60 by BNAH acts as an electron donor to O2 to give O2˙− in NMP.

Graphical abstract: Direct detection of superoxide anion generated in C60-photosensitized oxidation of NADH and an analogue by molecular oxygen

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Article information

DOI
https://doi.org/10.1039/B206887G
Article type
Paper
Submitted
15 Jul 2002
Accepted
06 Aug 2002
First published
26 Sep 2002

J. Chem. Soc., Perkin Trans. 2, 2002, 1829-1833

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Direct detection of superoxide anion generated in C60-photosensitized oxidation of NADH and an analogue by molecular oxygen

I. Nakanishi, K. Ohkubo, S. Fujita, S. Fukuzumi, T. Konishi, M. Fujitsuka, O. Ito and N. Miyata, J. Chem. Soc., Perkin Trans. 2, 2002, 1829 DOI: 10.1039/B206887G

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